Infertility has become a challenge in many mammalian species. In humans, about 15% of couples fail to conceive within the course of a year of unprotected sex. Within the United States, 11.0 % of women ages 15-44 have impaired fecundity and about 6% are infertile. Assisted reproductive technologies have become well developed and utilized to overcome some of the challenges of infertility. However, assessment of embryo quality and potential of in vivo and in-vitro produced embryos is largely based on morphology which is often not indicative of the embryo's ability to establish a pregnancy. Thus, the objectives of my research are to identify and characterize genes and epigenetic markers as predictors of embryo development using non-invasive methods. We are investigating the roles of imprinted genes and epigenetic modifications in early embryonic development. Another focus of my research is the investigation of the effects of maternal nutrition on the transcriptome and epigenome of the offspring. Methods used in our lab include genome-wide association studies, RNA-Seq, DNA methylation, CRISPR, microRNAs, and RNAi.